Figure 4

Example of the relationship between environmental factors and organization of regulatory potential in Acid Mine. A) Seasonal variation in pH, conductivity and iron concentration across the year. The left panel represents samples from “UBA” and right panel represents the samples collected from “5WAY” (data taken from [29]). B) TFBSs found in the promoters which regulate Ton and Tol transport system genes. The left panel shows a graphic illustrating the regulatory potential related to Ton and Tol transport system in Acid Mine (“UBA”). The promoters are grouped by function and species (X axis). Each bar in the graphic represents a promoter and, the red color in the bars indicates that the number of sites per promoter is equal or more than fifteen. Below are represented the most abundant palindromic sites found in those promoters, some of them corresponding to known TF, such as, LexA, ModR, AgaR, etc. The right panel represents a network of TFBSs shared between promoters of TonB dependent receptor and iron chelator utilization protein; both proteins are represented by white circles. The TFBSs are represented by colored circles with size proportional to the number of sites found.