Figure 3

Exon expression and gene expression. Calculation of exon expression versus gene expression using the standard splicing index and the residual regression score proposed in this work. (A) Plot of the exon expression signal versus the gene expression signal of gene RGN measured in 11 human tissue samples by exon microarrays (3 replicates per sample). The exon signal is calculated using the probes that map to the specific exon ENSE00001527616 and the gene signal is calculated using the probes that map to the common conserved exons included in the long transcripts of the gene. A linear regression is applied to the data to calculate the expression of the gene-exon pair. The 3 samples corresponding to cerebellum (labeled as “cer”) are placed in a circle to show that the average expression of these samples shows a significant deviation from the linear regression calculated for the whole gene. (B) Expression signal profile of gene RGN (ENSG00000130988) across the 33 samples (red line with dots) compared to the profile of exon ENSE00001527616 (black line) and the profiles of the rest of the exons (grey lines). (C) Plot of the residual regression scores calculated for each sample for the gene-exon pair ENSG00000130988-ENSE00001527616. (D) Plot of the splicing indexes calculated for each sample for the gene-exon pair ENSG00000130988-ENSE00001527616.