Figure 2

Performance metrics for all tested WGA methods. A) Call rate, the proportion of loci where a call was provided, correct or not. B) Error rate, split into the three possible categories of errors: allele drop-out (AB- > AA), heterozygosity gain (AA- > AB) and homozygous reversal (AA- > BB). The error rate is calculated relative to the number of calls provided by the method. Here error bars represent the standard deviation of the overall error rate, and not of individual types of errors. C) Proportion of correct calls, or loci where the method provided the correct genotype. A “no call” where the reference provided a genotype is considered a failure to provide the correct call. Error bars represent one standard deviation. Bars with different letters represent data that are significantly different (p<0.05), as determined by Post-hoc analyses using Games-Howell test. LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.