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Figure 3 | BMC Genomics

Figure 3

From: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates

Figure 3

Yield of amplified gDNA using different combinations of commercial gDNA extraction kits and WGA technologies. When starting with the recommended minimal input (i.e. 10 ng), all kits performed well, however SPIA-based WGA systems produced the most high quantity outputs than the MDA-based WGA systems. Whereas with gDNA obtained from 15 cells, only the REPLI-g Mini kit (MDA-based WGA) and Quick gDNA MicroPrep-Ovation WGA System (SPIA-based WGA) offered the best amplified gDNA output. Error bars represent one standard deviation. Bars with different letters represent data that are significantly different (p<0.05), as determined by Post-hoc analyses using Games-Howell test. MDA: multiple displacement amplification; SPIA: single-primer isothermal amplification.

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BMC Genomics

ISSN: 1471-2164

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