Figure 1

The ribosome profiling system. A) Diagram of work flow. B) Visualization of the sequence mapping onto the genome in Artemis. This image shows the spliced leader, ribosome footprint and mRNA reads mapping to the region of the STOP axonemal protein gene in slBF. Reads are color-coded as shown below the image; data for expression in slBF are shown in purple-pink throughout the manuscript. Here and elsewhere, start codons are shown in pink in the three reading frames while stop codons are black. The numbers under the stacked reads correspond to the coordinates in the chromosome and the GeneID for TriTrypDB is shown. C) SL, ribosome profiling, and mRNA reads mapping to the region of a DEAH box helicase gene in PCF. This gene is one of only two genes in the T. brucei genome that has an intron. Note the lack of ribosome profiling reads in the intron even though a low level of mRNA is present. Data for expression in PCF are shown in blue-green colors throughout.