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Figure 3 | BMC Genomics

Figure 3

From: Extensive stage-regulation of translation revealed by ribosome profiling of Trypanosoma brucei

Figure 3

Ribosome profiling reveals extensive differential protein production. In this smear plot the fold change in read counts for ribosome footprint (A) and mRNA (B) were plotted against average read counts per million reads of the pooled libraries for slBF and PCF. Dots that lie outside the blue lines are up-regulated at least 2-fold. Those that are statistically supported (FDR ≤0.01) are colored (green/dark green for PCF and pink/magenta for slBF). Note that almost twice as many genes (2971) up-regulated for protein production as compared to mRNA expression (1589). C) Stage-regulation of genes most highly expressed at the level of protein production. This dot plot depicts the gene rank for protein production in slBF and PF. The rank is based on median ribosome footprint RPK in the biological replicates. Those in the top 5% for slBF are outlined in magenta, those in the top 5% for PCF are green, and those that are in the top 5% for both appear purple. The remaining genes are marked in gray. D) Categorization of most highly expressed genes compared to genome-wide representation. Top 5% of slBF, magenta; top 5% PCF, green; genome, black.

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