TruSeq stranded | Encore complete | Ovation RNA-Seq V2 | SMARTer stranded | |
---|---|---|---|---|
RNA input (minimal requirement according to the manufacturer) | 100 ng depleted RNA | 100 ng total RNA | 0,5 ng depleted RNA | 1 ng depleted RNA |
Minimum quality | FFPE | RIN >7 | FFPE | FFPE |
rRNA depletion required | Yes | No | Yes | Yes |
cDNA synthesis | Random primers | Selective priming | Random and oligo(dT) primers | Random primers |
Fragmentation method | RNA by divalent cations + heat | cDNA by Covaris shearing | cDNA by Covaris shearing | RNA by heat |
Strand selection | Yes | Yes | No | Yes |
Library preparation method and reagents | Included | Included | Not included | Included |
Multiplex capacity | 96-plex | 16-plex | according to the library preparation method chosen | 12-plex |
Experiment duration | 6 hours | 7 hours | 4.5 hours for cDNA synthesis | 4.5 hours |
+ time for library preparation |