Figure 5

CG enrichment and methylation at potential promoters of TaMET1 genes from homoeologous group 5 and 7. A) Frequency of CG dinucleotides. Frequencies were computed every 50 bp and are shown for each homoeologous group. Putative promoter region and coding sequence are delimited by respectively a blue and white box. Black bars numbered from PCR1 to PCR4 highlight the four regions studied by bisulfite sequencing and are indicated above the graphs. Region 4 is specific to the 7D homoeolog. Arrows indicate the putative transcription start site according to the RNA-seq data. B) Mean values of CG dinucleotides. Mean values of the number of CG dinucleotides of the three homoeologs (A, B and D) for a given homoeologous group (2, 5 and 7) in the putative promoter (blue) and coding (white) sequence regions. Differences between groups 5 and 7 putative promoter regions and group 2 are indicated above the histogram. Statistical significance was confirmed with a Kruskal Wallis non parametric tests with *: P < 0.05; **: P < 0.01; ***: P < 0.001. C) DNA methylation profiles as determined by bisulfite sequencing. Percentages of methylated cytosines of the four amplicons (PCR1 to 4) displayed in Figure 6A were determined after bisulfite sequencing. Percentages of methylation were recorded at each cytosine position and were used to compute a mean value for each amplicon in the CG, CHG or CHH sequence contexts.