Figure 1

Expression profiles of cell wall modifying proteins across the Globodera pallida life cycle. Life stages indicated on X axis, Y-axis indicates reads per kilobase per million. Each line represents the expression profile for an individual gene. (A) Expansins: Expression profiles of nine sequences are shown. GPLIN_000293700 (orange line), GPLIN_000536200 (darker green line) and GPLIN_000599100 (light blue line) are upregulated at J2 and adult male stages while the other six sequences (GPLIN_000092400, GPLIN_000293400, GPLIN_000590900, GPLIN_000599200, GPLIN_001571600 and GPLIN_001621500) are only weakly expressed at the life stages tested; (B) Cellulases: Expression profiles of fifteen sequences are shown. One sequence GPLIN_000536400 – dark blue) is upregulated at J2 and male, five sequences (GPLIN_001111200 – purple, GPLIN_001111300 – light blue, GPLIN_001215600 – light olive, GPLIN_000313600 – pink and GPLIN_000552400 – brick red) are upregulated at J2, two sequences (GPLIN_000755200 – orange, and GPLIN_000755100 – light blue) are upregulated at male only. The remaining cellulase-like sequences (GPLIN_000616300, GPLIN_000694900, GPLIN_000779000, GPLIN_779200, GPLIN_000827200, GPLIN_001185800 and GPLIN_000304900) are weakly expressed at the life stages shown here; (C) Pectate Lyases: Expression profiles of seven sequences are shown. Three sequences (GPLIN_000467400 – blue line, GPLIN_000142600 and GPLIN_000412300 – overlaying graphs appearing green in the figure) are upregulated at J2 while four others (GPLIN_000673000, GPLIN_000294400, GPLIN_000294500 and GPLIN_000322300) are weakly expressed at the stages shown here; (D) Carbohydrate Binding Modules; (E) GH53 arabinogalactan endo 1,4 ß galactosidases. For Figure 1E the left hand Y axis shows scale of expression for GPLIN_000142900 (blue line) while the right hand Y axis shows scale of expression for GPLIN_000143000 (red line).