Figure 4

Alignment of contigs with sequences that could potentially be derived from food. Selected BLAST hits aligned using MAFFT and visualised with Jalview, coloured by BLOSUM62 score. A) Contig 2129 exhibits complete identity across the kingdom Viridiplantae 28S rRNA. Alignment with potential dietary plant and meat foodstuffs and the human rRNA gene. B) Contig 2062 is very similar to many chloroplast rRNA sequences and is shown aligned to several of the best hits and potential dietary sources. 1 - Pseudendoclonium akinetum: 2 - Trichosarcina mucosa: 3 - Lycopodium clavatum: 4 - Zygnema: 5 - Solanum Lycopersicum: 6 - Solanum tuberosum: C) All the lineages to which Contig 1748 has a perfect match, including many potential food sources. Representative sequences from each species are aligned and coloured by percentage identity (1: Fragaria vesca, 2: Medicago truncatula, 3: Lotus japonicus, 4: Glycine max, 5: Arabidopsis thaliana, 6: Solanum lycopersicum, NB: A 30 bp insertion present in Glycine max immediately 5 prime of the contig 1748 sequence is omitted to facilitate visualisation). Full lineage of core eudicotyledons is [root; cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; eudicotyledons].