Figure 3
From: Identification and functional characterization of copy number variations in diverse chicken breeds
Validation through qPCR in five test chicken breeds. Twenty-five samples from five breeds, Gushi chicken (GS), Lushi chicken (LS), Silkie chicken (SK), Xichuan Black-bone chicken (XC) and Houdan chicken (HD), were analyzed in qPCR for the six loci. The six loci are THRSP locus (A), PCCA locus (B), PRLR locus (C), SOCS2 locus (D), RHACD8 locus (E) and EDN3 locus (F), respectively. Each DNA sample was diluted to 10 ng/μL, and the concentrations were verified using a spectrophotometer. Quantitative PCR analyses were processed using a standard curve method as previously described [32].