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Table 1 RNA sequencing results

From: RNA sequencing provides evidence for functional variability between naturally co-existing Alteromonas macleodii lineages

Strain

AltDE

AltDE1

Growth Conditions

RMa-13°C

RMa-25°C

MMGb-25°C

STRc-25°C

RMa-13°C

RMa-25°C

MMGb-25°C

STRc-25°C

Total number of reads

3,444,176

11,503,336

8,986,648

2,995,620

6,230,679

7,840,241

13,514,524

14,695,977

Reads mapped to ribosomal operons

3,206,468

10,852,030

8,764,784

2,940,326

5,812,308

7,342,658

13,314,002

14,668,090

Percentage of reads mapped to ribosomal operons

93.1

94.3

97.5

98.2

93.2

93.7

98.5

99.8

Chromosome length (nt)

4,480,937

4,643,844

Average coverage per nucleotided

8.7X

14.8X

5.0X

1.2X

8.9X

10.5X

4.1X

0.6X

Percentage of CDS expressed

76.9

90.3

86.5

86.2

82.7

88.8

87.6

43.7

Reads mapped in the chromosome

387,708

661,306

221,864

55,294

412,700

487,487

191,820

27,243

Reads mapped to CDS

258,240

454,504

160,098

40,566

275,125

325,206

139,930

21,824

Percentage of reads mapped to IR

33.4

30.2

27.8

26.6

33.3

33.3

27.1

19.8

Percentage of reads mapped to ribosomal proteinse

21

22.9

11.2

6

20.9

20.1

4

1.2

Plasmid length (nt)

--

--

--

--

303,282

Average coverage per nucleotide

--

--

--

--

1.9X

3.3X

2.9X

0.2X

Reads mapped to plasmid

--

--

--

--

5,671

10,096

8,702

644

  1. CDS, coding DNA sequence; IR, intergenic region.
  2. anutrient rich marin medium.
  3. bminimal medium with 1% glucose.
  4. cminimal medium with 1% glucose followed by two days of minimal medium without glucose.
  5. dnumber of mapped reads X 100/number of nucleotides in the chromosome.
  6. epercentage of CDS.