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Figure 5 | BMC Genomics

Figure 5

From: Development and bin mapping of gene-associated interspecific SNPs for cotton (Gossypium hirsutum L.) introgression breeding efforts

Figure 5

KASP genotyping of marker UCcg10563_649. A1 quadrant of a 384-well plate KlusterCaller image of genotyping KASP assay for marker UCcg10563_649 after 38 cycles. A.) G. barbadense L. (AD)2 cv. 3–79, B.) G. hirsutum L. (AD)1 line TM-1, C.) F1 euploid hybrid G. barbadense L. (AD)2 cv. 3–79 and G. hirsutum L. (AD)1 line TM-1, D.) F1 hypo-aneuploid lines for AD-Chromosome 17, E.) Wide-cross whole-genome radiation hybrid samples, X.) Non-template (negative control), in B2 quadrant (not shown). Both samples in D and 6 of the samples in E show deletions due to the shift in genotype from F1-green to homozygous for the G. barbadense L. (AD)2 allele – blue. [The two samples shown in white and yellow were considered questionable genotypes as they did not fall directly in a cluster].

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