Figure 7
Network characteristics and sequence conservation of the age-associated DNA methylation signature. (A) Protein interactions of age-associated DNA methylation genes in normal tissue. Blue or green nodes indicate genes hypo- or hypermethylated with age; gray nodes indicate the interacting neighbors of the age-associated gene products. Red or yellow borders of nodes indicate whether the loci are located in CGIs (red) or non-CGIs (purple). Edges between nodes indicate the protein–protein interactions of gene products. Node sizes are proportional to numbers of interacting proteins. (B) The distributions of numbers of interacting protein neighbors from the network shown in (A) (in red), or from all protein interactions combined (in blue), respectively. (C) The number of direct protein interactions between gene products affiliated with the age-associated DNA methylation signature is indicated by a red arrow. The black curve indicates the distribution of 106 random selections of proteins with the same number of the age-associated genes. (D) The number of the age-associated DNA loci with significant the average phyloP scores (>1.3) is indicated by a red arrow. The black curve indicates the background distribution of the human genome. (E) Surrounding sequences of age-associated loci in MYF5 and MYF6.