Figure 6

Expression patterns of seven miRNAs in secretory- and maturation-stage enamel organs as shown by in situ hybridization. (A) Negative control. Samples were incubated without any miRNA detection probes. (B) Positive control. U6 detection probes were used for incubation. (C) Scrambled control for secretory-stage enamel organ. (D) Scrambled control for maturation-stage enamel organ. (E), (G), (I), (K), (M), (O) and (Q) The expression of miR-21, miR-31, miR-488, miR-153, miR-135b, miR-135a and miR-298 in secretory-stage enamel organ. MiR-21, miR-31 and miR-488 are down-regulated, while miR-153, miR-135b, miR-135a and miR-298 are up-regulated in secretory-stage enamel formation compared to maturation-stage. (F), (H), (J), (L), (N), (P) and (R) The expression of miR-21, miR-31, miR-488, miR-153, miR-135b, miR-135a and miR-298 in maturation-stage enamel organ, showing altered maturation-stage expression patterns. Images shown at 20× magnification. S, secretory stage. M, maturation stage.