Figure 7

Lamp1 is the potential target of miR-153. (A) The expression levels of miR-153 in LS8 cells before and after transfection with miR-153 mimics (blue) and inhibitors (red). (B) Relative luciferase reporter activities in luciferase reporter assay validating the interaction of miR-153 with 3′-UTR of Lamp1. Luciferase activities are presented in ratios of Firefly (F) to Renilla (R) luciferase reporter activities. For the data presented, the amount of luciferase reporter vector used was 700 ng, and the concentrations of miR-153 mimics and inhibitors in final transfection complex were 20 pM and 0.2 nM, respectively.