Figure 8

Tfrc is the potential target of miR-31. (A) The expression levels of miR-31 in LS8 cells before and after transfection with miR-31 mimics (blue) and inhibitors (red). (B) Relative Luciferase reporter activities in luciferase reporter assay validating the interaction of miR-31 with 3′-UTR of Tfrc. For the data presented, the amount of luciferase reporter vector used was fixed at 700 ng, and the concentrations of miR-31 mimics and inhibitors in final transfection complex were 20 pM and 0.2 nM respectively.