Figure 2
From: Adenine methylation may contribute to endosymbiont selection in a clonal aphid population
Isolation of methyladenine genomic fragments using methyl-sensitive restriction enzymes, followed by PCR amplification. DNA fragments containing methylated adenines were isolated from samples of total genomic DNA of A. pisum using the methylation-site-specific restriction enzyme DpnI, adaptor ligation and the methyl-sensitive restriction enzyme DpnII. This was followed by PCR amplification as described by Steensel and Henikoff [59]. The resulting PCR products were cloned and sequenced.