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Table 2 Summary of the adenine methylation on the secondary endosymbiont’s helicase gene

From: Adenine methylation may contribute to endosymbiont selection in a clonal aphid population

Primers

 

A6F3 & A6R2

 

A6F1 & A6R1

 

A6F2 & A6R2

 

Phenotype

*No. of aphids

DpnI

DpnII

DpnI + DpnII

DpnI

DpnII

DpnI + DpnII

DpnI

DpnII

DpnI + DpnII

22°C ( Orange )

6

(6)+++

(6)+++

(6)+++

(6)+++

(6)+++

(6)+++

(2)++(4)+

(6)+++

(2)++(4)+

8°C ( Green )

6

(6)+++

(6)+++

(6)++

(3)++(2)+

(6)+++

(3)++(3)+

(3)++

(6)+++

(2)+

  1. *Number of individual Acyrthosiphon pisum aphids tested by PCR amplification. The amplicon intensity was categorized into three levels: +++, high; ++, medium; +low; -, no amplification.