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Figure 3 | BMC Genomics

Figure 3

From: An improved ChIP-seq peak detection system for simultaneously identifying post-translational modified transcription factors by combinatorial fusion, using SUMOylation as an example

Figure 3

Confirmation of ChIP-seq data for ELK1-binding sites with SUMO-1 enrichment in BCBL-1 cells using ChIP-qPCR. Confirmation of data derived from ChIP-seq for ELK1 binding sites with SUMO-1 enrichment in BCBL-1 cells. The ELK1 binding sites within the SUMO-1 peak of the promoters of (A) TARS2, (B) NDUFB7 and (C) ADAMTS10 genes were amplified using qPCR. (D) SNRPE, (E) INO80B and (F) LYSMD1 genes identified in our SUMO-1 ChIP-seq result and GSM608163 ChIP-seq data were analyzed by qPCR with their specific primer pairs. All reactions were run in triplicate and normalized against the input. Nonspecific IgG was used as the control ChIP antibody.

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BMC Genomics

ISSN: 1471-2164

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