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Figure 4 | BMC Genomics

Figure 4

From: An improved ChIP-seq peak detection system for simultaneously identifying post-translational modified transcription factors by combinatorial fusion, using SUMOylation as an example

Figure 4

Colocalization of ELK1 and SUMO-1 in the promoters of TARS2 and NDUFB7 genes. Sequential chromatin immunoprecipitation (ChIP-reChIP) assay using control IgG and anti-SUMO-1 antibody for the first ChIP and anti-ELK-1 antibody for the reChIP was performed in formaldehyde-fixed chromatin derived fromTREx-F3H3-K-Rta BCBL-1 cells. Quantification of first ChIP and reChIP DNA recovered from (A) TARS2, (B) NDUFB7 and (C) SNRPE, (D) INO80B and (E) LYSMD1 by real-time qPCR using the promoter-specific primers.

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BMC Genomics

ISSN: 1471-2164

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