Figure 5
From: TIGAR2: sensitive and accurate estimation of transcript isoform expression with longer RNA-Seq reads
Performance evaluation with TIGAR2, TIGAR1, RSEM, and Cufflinks using paired-end simulation data. Root mean square errors of the predicted transcript isoform abundances with each method against the true gene expression levels are shown for 100 bp, 250 bp, 500 bp, and 1,000 bp paired-end simulation data. Because RSEM and TopHat-Cufflinks did not produce predictions for 500 bp and 1,000 bp paired-end reads, errors were calculated assuming abundances were estimated as zero for all isoforms.