Figure 5

Metabolic flux map of central carbon metabolism in cotyledons at early seed fill. Glycine max, var. Evans plants were grown in a growth chamber. Seeds were harvested at 21 DAF and cultured in medium containing ¹³C-sucrose until 26 DAF. Widths of flux arrows are drawn in proportion to relative fluxes; arrow directions indicate net flux. Each flux is represented by the metabolic reaction, with a superscript indicating subcellular localization (if known): c, cytosol; p, plastid; and m, mitochondrion. Oval fill colors: intracellular metabolites (white); "sink" metabolites such as proteinogenic amino acids or polysaccharides (gray); metabolites absorbed from/discharged into the medium (blue). A. Metabolic flux map. Flux arrow and name colors: glycolysis and sucrose metabolism (dark red); pentose phosphate pathway (pale blue); TCA cycle (orange); pyruvate dehydrogenase (purple-gray); anaplerotic reactions (mauve); glyoxylate shunt (green yellow); glucose metabolism, GABA shunt and related transmembrane fluxes (brown); fluxes towards synthesis of biomass (gray); all other transmembrane fluxes (black). B. Integration of metabolic flux with transcriptomics data. Flux arrow and font colors: reactions that have transcript support for encoded proteins predicted to be in the same subcellular localization as the reaction (dark green); transcripts for encoded proteins of undetermined subcellular location (light green); no transcript identified from the soybean genome and/or and no transcript on the Affymetrix soybean genome chip, and/or multi-step reactions (grey).