Mutation screening of two candidate genes from 13q32 in families affected with Bipolar disorder: human peptide transporter (SLC15A1) and human glypican5 (GPC5)

Table 3 Human peptide transporter (SLC15A1) : PCR Primers and Annealing temperature

Exon	cDNA bp	Position	Forward Primer Primer sequence 5' → 3'	Reverse Primer Primer sequence 5' → 3'	Product Size bp	Annealing Temp.
2	61–77	P17	ccctctgaccaccctaaaaa	tgaacccttaggggtaaaaca	191	60
3	78–159	P1	tggggaaggattagtgtaggg	aactttcccagccacgagt	498	60
4	158–302
5	302–422	P2	tgtggtggagtcaaaagtgg	cctgaagacccagctcaatc	356	60
6	422–522	P3	gtcactatgccaggcccact	gcctctgactcctggatgtg	258	60
7	519–611	P18	agtggattgatagccaaagtcatct	aagacacggacttggcctta	200	60
8	611–696	P4	tgtgaaagcaatacgtaattatcag	ctactttttgttgccacttgttacat	295	60
9	697–779	P16	tcaagagccatttctattcttcc	gcatctctctaggccacagg	350	48
10	780–867	P5	tgaaatgtgcttccctgaca	tcactgaccattttgtccatgt	254	60
11	867–957	P6	acctcccagcttgcctcta	tgaagtgagccttggtacctg	293	60
12	955–1002	P7	cagggtactgctttgtgcag	tatcctctaggcgaggttgc	701	60
13	1002–1034
14	1030–1123
15	1124–1205	P8	aagatggggagaggtgcttt	gctccagggctcagtttaca	348	60
16	1206–1325	P9	aggtctgtgattggcagctt	gctggccttggcatttatac	379	60
17	1326–1476	P10	aaacctcatgacggtgtctg	ttttggcctccagtatcaca	400	60
18	1472–1522	P11	gcctccagagcccttctaat	tcagccagccttacattgct	331	60
19	1519–1630	P12	gacattgtggcggaatctct	gggagtattgcccaacttca	356	60
20	1631–1739
21	1738–1884	P13	gtcccatcagcattttctgc	ggtcaaactcaatttacctgttcg	292	60
22	1879–1991	P14	ccatgatgaccatgaacagg	cattgaggccacctgacttt	299	60
23	1993–2315	P15	ccaagaacatgtacgcacca	aggctgaggcaggagaatta	540	60

ISSN: 1471-2164