From: Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis
Protocol
Affymetrix
Affymetrix w/o ligase
Number of amplifications
6
5
Correlation coefficient
Virtual
0.84
0.86
Average
0.79 ± 0.04
0.82 ± 0.04
Yield of aRNA (μg)
24.1 ± 4.7
19.2 ± 5.9
Fold of amplification
803 ± 157
640 ± 197