Table 3 Fusions to B42 that are unstable in E. coli
Library | Insert | Comments |
|---|---|---|
E. coli | YdaW | putative membrane protein |
| Â | YaaU | probable transporter |
| Â | PhoQ | transmembrane receptor |
| Â | YjcE | putative Na+/H+ antiporter |
| Â | GalR | gal operon repressor protein |
| Â | YicE | hypothetical xanthine/uracil permease |
| Â | PrfC | polypeptide release factor 3 |
B. subtilis | YwtA | polyglutamate biosynthesis |
| Â | YvaL | EcSecG homolog, peptide secretion |
| Â | MreB | Bacterial actin homolog, cell shape determination |
| Â | YwdJ | Ipa-60d, homology to purine permeases |
| Â | TrpB | Tryptophan synthase, beta subunit |
| Â | CotS | Spore coat protein |
| Â | Mfd | Removes RNA pol from DNA lesions |
| Â | YrvE | Homology to ssDNA exonuclease |