Figure 1From: Microarray analysis of tumor necrosis factor α induced gene expression in U373 human glioblastoma cellsNorthern Blot analysis of IκB-α in U373 cells after TNF treatment (A) or PDTC and TNF cotreatment (B). U373 cells were stimulated with 10 ng/ml TNF-α for the time indicated. RNA was blotted onto a nylon membrane after gel electrophoresis. Hybridization was done with probes for IκB-α and GAPDH as a loading control. Note that the induction of IκB-α by TNF is blocked by cotreatment with PDTC.Back to article page