Table 2 Haploid DNA copy number in IMR-32 and SK-N-SH compared to a normal human control sample and fold expression difference between IMR-32 and SK-N-SH (tester vs. driver): Real-time quantitative PCR based determination of gene copy number and fold-expression of 5 previously reported amplified genes (MYCN, DDX1, NAG, NSE1, MEIS1) and 6 other amplified 2p clones (rounded mean of 2 measurements) (*primers designed in 2p24.3; **no expression in SK-S-SH).
GENE | CLONE | ACCESSION NUMBER | LOCATION | HAPLOID COPY NUMBER IN IMR-32 | HAPLOID COPY NUMBER IN SK-N-SH | FOLD OVER-EXPRESSION |
|---|---|---|---|---|---|---|
MYCN | NM_005378 | 2p24.3 | 53 | 1.26 | 513 | |
DDX1 | NM_004939 | 2p24.3 | 42 | 1.28 | 40 | |
NAG | AF388385 | 2p24.3 | 41 | 1.31 | 37 | |
NSE1 | g10e3 | CD664581 | 2p24.3 | 70 | 1.27 | 280 |
g9d9 | CD664576 | 44 | 1.05 | 193 | ||
NM_145175 | 34 | 0.93 | 236 | |||
- | g10d12 | CD664582 | 2p24.3 | 27 | 1.17 | 1616 |
TEM8 | g1h7 | CD664531 | 2p13.3 | 26 | 1.02 | 8 |
- | g4d5 | CD664538 | 2p14 | 30 | 1.04 | 1819 |
MEIS1 | g6f6 | CD664530 | 2p14 | 42 | 1.27 | 10 |
- | g2h10a | CD664534 | 2p24.3* | 42 | 1.07 | ** |