Figure 4

Empirical Distributions of the Detection p -values Detection p-values (x-axis) are plotted against the empirical cumulative probability (y-axis) of values less than those on the corresponding curve. Plots shown in (A) and (B) represent data from normal cell line and those in (C) and (D) correspond to cancer cell line. The gap between curves in the plots shown in (A) and (C) corresponds to about 10% of the genes that were detected using the IVT but not the dIVT protocol. The near coincidence of graphs in (B) and (D) shows that virtually all genes detected using the dIVT protocol are also detected using the IVT protocol.