Table 1 Retention Studies on Commercial Coated Slide Surfaces
From: Immobilized probe and glass surface chemistry as variables in microarray fabrication
Vendor | Chemistry | RFU/Pixel deposited × 103 | RFU/pixel retained × 103 | Percent Retention | Spot Diameter | Processed S/(S+N) |
|---|---|---|---|---|---|---|
MCW in-house | Poly-L-lysine | 19.0+/-7.7 | 11.1+/-2.7 | 58.4% | 114+/-9 | 0.90+/-0.00 |
Electron Microscopy Sciences | Poly-L-lysine | 15.3+/-4.9 | 2.12+/-1.1 | 13.9% | 100+/-28 | 0.85+/-0.04 |
Polysciences | Poly-L-lysine | 11.5+/-6.3 | 1.3+/-1.0 | 11.3% | 125+/-6 | 0.86+/-0.06 |
Cel Associates | Poly-L-lysine | 5.7+/-3.6 | 2.3+/-0.5 | 40.4% | 100+/-35 | 0.79+/-0.09 |
Asper Biotech | Aminated | 6.8+/-2.4 | 3.2+/-1.1 | 47.1% | 98+/-22 | 0.93+/-0.05 |
Apogent Ezrays | Aminated | 6.0+/-2.9 | 2.8+/-0.3 | 46.7% | 109+/-20 | 0.92+/-0.02 |
Bioslide | Aminated | 3.3+/-0.9 | 1.5+/-0.5 | 45.5% | 93+/-7 | 0.87+/-0.02 |
Erie Scientific | Aminated | 11.5+/-4.1 | 3.2+/-0.9 | 27.8% | 81+/-15 | 0.90+/-0.01 |
Genetix | Aminated | 4.1+/-1.2 | 1.2+/-0.2 | 29.3% | 94+/-23 | 0.86+/-0.02 |
Corning Ultra GAPS | Aminated | 2.3+/-0.3 | 1.5+/-0.2 | 65.2% | 103+/-13 | 0.92+/-0.01 |
Corning GAPS II | Aminated | 3.9+/-0.9 | 1.4+/-0.2 | 35.9% | 117+/-18 | 0.92+/-0.01 |
Sigma | Aminated | 20.7+/-3.3 | 5.4+/-1.0 | 26.1% | 93+/-8 | 0.79+/-0.01 |
Telechem Super Amine | Aminated | 6.7+/-2.1 | 1.7+/-0.5 | 25.4% | 107+/-19 | 0.80+/-0.0 |
Full Moon Biosystems | Proprietary | 6.4+/-1.4 | 2.1+/-0.2 | 32.8% | 78+/-7 | 0.90+/-0.1 |