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Table 1 Yield of RNA amplification generated from different cDNA purification methods.*

From: Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling

Method

28S/18S = 1.9

28S/18S = 1.4

column

18 μg ± 2

7 μg ± 1

ethanol at RT

17 μg ± 2

10 μg ± 1

isopropanol

19 μg ± 3

14 μg ± 2

ethanol at CT

14 μg ± 2

12 μg ± 1

  1. *RNA from MCF-7 and HCC-1187 (28S/18S = 1.9) and OCUB-M (28S/18S = 1.4) were used and reactions were repeated five times. Values represent averages with standard errors.
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BMC Genomics

ISSN: 1471-2164

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