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Table 9 Illustration of a nomenclature system

From: Proposed methods for testing and selecting the ERCC external RNA controls

Reagent

Nomenclature

Legend

RNA Transcript

ERCC-nnnnn-vv

nnnnn = unique 5-digit sequence number

vv = 2 digit version number

PCR primer/probe microarray probe

ERCC-nnnnn-vv- pppp-lll-aaa

pppp = 4-digit positional location relative to the 0th base at the 5' end of the transcript sequence

lll = primer/probe length

aaa = nucleic acid sequence of the initial triplet of the primer/probe (complement of the RNA sequence at pppp, pppp+1, pppp+2)