Table 1 Hybridization of single-copy gene probes on high-density membrane
Gene/Primer | GenBank accession/Primer sequence | Genomic position1 | Length | Positive clones |
|---|---|---|---|---|
Mash | NM_008554 | |||
mMashCgi-F | ACCCGGTTCCTCGCGAGCACTTTTC | chr7:130,673,330 | 358 bp | 5/48 |
mMashCgi-B | AGCGCAGCGTCTCCACCTTACTCAG | chr7:130,672,998 | ||
Adseverin | NM_009132 | |||
CpG-Ads-1F | TCTTGGAGGGTCATACTCATT | chr12:35,153,275 | 516 bp | 7/48 |
CpG-Ads-1R | GCAGCTCAAAATAATTACGAC | chr12:35,152,780 | ||
Igf2r | NM_010515 | |||
Igf2r-H4F | TCAGAACACTGGTGAGCAGTGGG | chr17:12,150,732 | 244 bp | 11/48 |
Igf2-H4R | GAGGGTAGGATTCCGTTGCAAGG | chr17:12,150,509 | ||
Tbp | NM_013684 | |||
* probe derived from tbp-1942 clone | chr17:14,324,440 | 1085 bp | 4/48 | |
chr17:14,334,524 | ||||
Usp26 | NM_031388 | |||
Usp26-A | AATGTAACGAAGGGAGAAGTG | chrX:44,101,298 | 206 bp | 3/48 |
Usp26-B | AGGCTTTGCCTTCTTATCGAG | chrX:44,101,113 | ||
Xist | AY618354 | |||
mXistF | AGTGGGTGTTCAGGGCGTGG | chrX:94,885,925 | 293 bp | 11/48 |
mXistR | CTATCCCCTAGTCCTCTGCGG | chrX:94,885,652 | ||
Tex13 | NM_031381 | |||
Tex13 pub-1F | ACCAGAGTTGGGAACAACTAA | chrX:130,816,501 | 220 bp | 7/48 |
Tex13 pds-1R | CTGTTGTAGAGGGTAGAGGTT | chrX:130,816,302 |