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Table 2 Estimation of subproteome study error rate.

From: Assessing the precision of high-throughput computational and laboratory approaches for the genome-wide identification of protein subcellular localization in bacteria

Organism

Fractiona)

Total proteins identified

Disagreementsb)

Confirmed PSORTb errorsc)

Confirmed laboratory errorsd)

% Errorse)

E. coli [64]

C

23

1

0

0

0.0

Synechocystis [15]

CM

63

25

0

4

6.3

Synechocystis [46]

P

57

5

0

1

1.8

K. pneumoniae [16]

OM

3

0

0

0

0.0

S. typhimurium [16]

OM

11

2

0

2

18.2

E. coli [17]

OM

39

8

0

6

15.4

P. gingivalis [18]

OM

6

1

0

1

16.7

P. aeruginosa [13]

OM

33

6

0

3

9.1

P. aeruginosa [13]

EC

150

81

2

36

24.0

H. pylori [19]

EC

20

9

1

5

25.0

Total

 

405

138

3

58

14.3

  1. a) C = cytoplasmic, CM = cytoplasmic membrane, P = periplasmic, OM = outer membrane, and EC = extracellular.
  2. b) Disagreement represents the number of proteins of the fraction X predicted by PSORTb not to be resident at X or X/Y localization sites.
  3. c) Confirmed PSORTb error represents the number of disagreeing cases for which the PSORTb predicted localization site was found to be incorrect.
  4. d) Confirmed laboratory error represents the number of disagreeing cases for which the PSORTb predicted localization site was found to be correct.
  5. e) % Errors is calculated as the number of confirmed laboratory errors divided by the total number of proteins identified.