Table 3 PCR primer sequences used to amplify DNA (D) or cDNA (C) templates for mutation screening. The order of the amplicons listed is the same as in Table 1.
From: Efficient gene-driven germ-line point mutagenesis of C57BL/6J mice
Gene symbol | Amplicon size (bp) | Template | Forward primer (5' to 3') | Reverse primer (5' to 3') |
|---|---|---|---|---|
Mc1r | 579 | D | gaggatccttcctgacaagactatgtcca | aacggctgtgtgcttgtagtagg |
Mc1r | 446 | D | tcgtctccagcaccctctttatc | gagtcgacgatcaccaggagcacagcagca |
Mc1r | 527 | D | gcacacttctaatggagagtg | ggctcaggtagagacatgcc |
Zfp111 | 352 | D | ccagagagaaataatgcccc | caagaaccctgggctctcc |
Zfp111 | 327 | D | ggttcagtcaggtctcacac | caggattgatataatgctcc |
Scnm1 | 394 | D | aggcacagcgtctcaaagtattgt | atagggtagtaggggtcaccactc |
Scnm1 | 484 | D | ggcaagaagcatttgtccagtaag | tttctcagaatacaatctggagagc |
Scnm1 | 372 | D | tgagttcccgtcagccaggacttc | ctcactcaccttcggagggtaagg |
Usp29 | 300 | D | ccttactcatcgacaagttatc | tggaggaggatggttctgtctt |
Usp29 | 336 | D | gggtctgctggcaccaaaaggt | ggcaacaagtcagtggtaact |
Usp29 | 298 | D | gggtcttcaagaggttccagag | gagcctgtaattctgaagatca |
Zim1 | 298 | D | ggaagaagacaggggataattcc | ggagcgctctgtggtgttgtag |
Zim1 | 298 | D | atcttcgggtcaaacatcagca | gtagtgtgtgaggaagtatgaga |
Zim1 | 302 | D | tggagagtgtaacaagtgcttc | ggtgcttgagaagggctactttg |
Zim1 | 302 | D | cccggagtgtgggaaagtcttc | ggtgaatcagcagggtagccagt |
Myd88 | 429 | D | ggctggcaggagacttaagg | caggaagcacgtttcctcac |
Myd88 | 196 | D | cacccttctcttctccacag | gcccacctattctacctagg |
Myd88 | 497 | D | ccttctgcagaggctgattg | ccaaagcaggcctaagcttac |
Myd88 | 245 | D | cgtggtcctaataccacacc | ggaggcaagcggaagaacac |
Ap2s1 | 424 | D | gccttgtctgtacctgtctc | ggacgagcaggcaggttggtc |
Capsl | 372 | D | gaggtaaacctagggcttctg | ctgccacgctgtcaataccg |
Capsl | 344 | D | gcacatgcatttctccatgg | gctttgtaaggctctgaacc |
Antxr1 | 419 | D | gagaatgggagatgaagttgg | gttcacctagcactttgtgg |
Antxr1 | 335 | D | catatggctgtcaacagcaagg | gagtgtcggttaaggagaag |
Antxr1 | 157 | D | gacgatctccaaagattcgg | gtaggactctgtggctgatg |
Antxr1 | 363 | C | gttctgccaggaggagacac | gcagatggtggatggttcag |
Antxr1 | 490 | C | aggctctccaaggcattatcc | ccatcatcgtcttcttcctcac |
Antxr1 | 489 | C | gctgctctggtggttctg | gtgttgttcaggggatacttg |
Ap2a1 | 510 | C | agaacgctatcctctttgagacc | ggacgtcatcacggttgg |
Ap2a1 | 543 | C | ggcttttgctgcagacattc | aggttgggctcactgtcatag |
Ap2a1 | 442 | C | gttgtcggtgcgcttcc | catataccagggcaagtccag |
Ap2a1 | 531 | C | ctatgtgagcgaggaggtgtgg | cggctgtcatctagggcactg |
Ap2a1 | 351 | C | agcgggagtcgtccatcttg | gagctgggtcagccaacaaag |
Pak4 | 503 | C | aacacatacccacgggctgac | cgcatgatcaccacctcattg |
Pak4 | 477 | C | gtacgcgggcacagagttc | ccgacatgttctcaaattcgtc |
Pak4 | 459 | C | aggatggggctctcactctg | cattaggggccatggtatgtg |
Pak4 | 412 | C | caagcagcaaagacgtgaaactg | atagggaaggcgggagatgag |
Pak4 | 447 | C | ctgtccgacttcgggttttgtg | gtagccaggctctttggttcaagac |