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Table 2 List of AMEV ORFs that were incorrectly classified.

From: Using purine skews to predict genes in AT-rich poxviruses

Major ORFS incorrectly classified as minor

ORF name

ORF size (bp)

Serine content (%)

Aspartate content (%)

Glutamate content (%)

Purine content (%)

Explanation

AMEV-161

243

11.11

2.47

1.23

47.56

Membrane protein

AMEV-164

708

7.63

2.12

2.97

47.97

High Ser%, low Asp% and Glu%

AMEV-171

276

3.26

1.09

1.09

48.39

Low Asp% and Glu%

AMEV-183

675

6.67

3.11

1.33

51.18

Low AG% and low Glu%

Minor ORFs incorrectly classified as major

ORF name

ORF size (bp)

Serine content (%)

Aspartate content (%)

Glutamate content (%)

Purine content (%)

Explanation

AMEV-152

225

0

12

1.33

60.97

Overlaps on same strand as major ORF

AMEV-189

180

1.67

8.33

1.67

43.17

Low Ser%, high Asp%

AMEV-191

228

0

2.63

10.53

61.9

Overlaps on same strand as major ORF