Figure 7

Detection of Spam1 transcripts by RT-PCR. Primer pairs specific for Gapdh, the Spam1 ORF, and Spam1 transcripts initiating within the ERV sequence were used in RT-PCR assays. The assays were carried out on cDNAs derived from a range of normal C57/BL6 mouse tissues: 1, brain; 2, heart; 3, kidney; 4, liver; 5, lung; 6, colon; 7, small intestine; 8, spleen; 9, stomach; 10, thymus; 11, muscle; 12, testis; 13, placenta. Approximate molecular weights are indicated on the left.