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Table 1 Comparisons of different methods used to identify IS5 neighbouring genes from microarray experiments. Results obtained when different test statistics (rank product – RP, median rank – MR, Mean, SAM and t-statistics) and normalization methods (global and scale normalization) were applied to experimental data from the control, isolate 'A' and isolate 'B'. These results were also compared to existing genome sequence data. Top 50 to 100 genes were predicted as IS5 neighbours from microarray experiments. The neighbours from sequence data were defined as 5 genes upstream and 5 genes downstream of the IS5 elements and IS5 itself, so there were 121 neighbours based on sequence data. The numbers in this table represent number of genes that overlap between the microarray experiment and the sequence data. The higher the number is, the better consistency between the microarray experiment and the sequence data.

From: Genome-wide localization of mobile elements: experimental, statistical and biological considerations

Method

Top 50

Top 100

  

control

A

B

control

A

B

Rank – based

RP

13

46

31

15

69

43

 

MR

13

45

32

15

70

47

Global Normalization

Mean

20

37

20

23

65

29

 

SAM

20

38

24

23

65

29

 

t

20

38

24

23

65

31

Scale Normalization

Mean

20

44

21

23

65

23

 

SAM

20

40

22

23

63

29

 

t

20

16

24

23

30

31