Table 1 BAC libraries fingerprinted for the tilapia physical map. Construction of these BAC libraries is described in Katagiri et al. [16]. Copies of the libraries are available as plates and filters from http://hcgs.unh.edu/BAC/.
Library | Cloning site | Vector | Mean insert size (kb) | No. of clones fingerprinted | Valid bands per clone | Genome coverage |
|---|---|---|---|---|---|---|
HCGS-03TI | HindIII | pBAC-lac | 145 | 18,700 | 53.9 | 2.56 |
HCGS-04TI | HindIII | pBAC-lac | 194 | 16,545 | 69.8 | 3.02 |
| Â | Total | Â | 182 | 35,245 | 61.4 | 5.58 |