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Figure 2 | BMC Genomics

Figure 2

From: Evidence for abundant transcription of non-coding regions in the Saccharomyces cerevisiae genome

Figure 2

RT-PCR analysis of dubious ORFs. A) Reverse-transcription was carried out using oligo dT and specific primers for each ORF. RT: reverse transcription followed by PCR amplification. N: No reverse transcriptase added. G: Genomic DNA (positive control). NS: No RNA or DNA substrate added (negative control). B) A similar RT-PCR analysis was carried out for sense-antisense pairs. Shown here are YGR181w/TIM13 and the DO YGR182c. No amplification was detected with primers p1 and p4. If a transcript was present that encompasses both ORFs, amplification was expected, of the size observed in the reaction carried out with genomic DNA

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