Table 1 Sequence and localization of the primers employed for the isolation ofthe midgut aminopeptidases from S. exigua by RACE experiments
Gene name | Primer sequence (5' to 3') | Position in ORF | |
|---|---|---|---|
APN1 | 3'Race | TGCGCTTCGAAGATTGGCTCACGATA | 2018–2044 |
|  | 5'Race | CTTCGATGAATGCCGAGACGCTGGAC | 2122–2097 |
APN2 | 3'Race | CTTTGCTGCTGGTGCTATGGAGAACTGG | 906–932 |
|  | 5'Race | ACAGGGCTGACTTCATTTCCGAACCATT | 1067–1040 |
APN3 | 3'Race | ATGCGTGAYGAYATGTACGGTAT | 496–518 |
|  | 5'Race | GCCAGGAGATAAGTTGACATTTTTGGGG | 779–752 |
APN4 | 3'Race | TGGCTTTCCATGTGAGCGATTTTGTGC | 728–754 |
|  | 5'Race | CACACCGATTTCTGCAGCATACGAGTGC | 849–822 |