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Table 6 The top 25 genes exhibiting increased mRNA abundance 16 hours post activation.

From: Development and validation of a bovine macrophage specific cDNA microarray

Clone

E value

Accession No.

Gene Symbol

Gene

Fold Increase

FDRmax

P value

pIL8

0.0

NM_173925

IL8

Interleukin 8

60.09

1.23E-18

4.91E-22

MARC_1BOV_94L3

0.0

NM_174300

CXCL6

Chemokine (C-X-C motif) ligand 6

27.46

1.72E-11

1.23E-13

C0006007m23

2.00E-72

XM_868409

DFNA5

Deafness, autosomal dominant 5

25.42

6.52E-13

2.72E-15

MARC_3BOV_60P17

0.0

NM_174299

CXCL2

Chemokine (C-X-C motif) ligand 2 (GROβ/MIP2A)

25.14

1.25E-15

1.24E-18

C0006006k10

0.0

XM_586036

ANKRD37

Ankyrin repeat domain 37

23.35

4.05E-07

1.51E-08

C0006016h15

0.0

XM_613370

RGS1

Regulator of G-protein signalling 1

22.10

5.10E-09

1.00E-10

C0006019g12

e-134

CK965770

-

EST

21.24

2.26E-11

1.80E-13

C0006019e20

0.0

NM_181015

PRNP

Prion protein

20.34

1.05E-20

2.08E-24

C0006014j10

6.00E-96

NM_000214

JAG1

Jagged 1

18.39

1.81E-08

4.42E-10

C0005208m7

e-110

NM_000785

CYP27B1

Cytochrome P450, family 27, subfamily B, polypeptide 1

18.39

4.10E-09

7.83E-11

C0006007a22

0.0

NM_022154

SLC39A8

Solute carrier family 39 (zinc transporter), member 8

16.01

7.46E-10

1.10E-11

C0005204j16

0.0

XM_864683

CXCL3

Chemokine (C-X-C motif) ligand 3 (GROγ/MIP2b)

15.28

1.20E-13

3.40E-16

MARC_2BOV_43M14

e-132

NM_001124

ADM

Adrenomedullin

14.23

1.20E-13

3.00E-16

C0005198l1

e-138

BI975933

-

EST

11.26

2.12E-05

1.30E-06

MARC_3BOV_120N21

e-102

NM_172219

CSF3

Colony stimulating factor 3 (granulocyte) (G-CSF)

11.14

8.11E-11

7.73E-13

C0006020e20

0.0

CB419326

-

EST

10.79

9.10E-12

5.60E-14

MARC_1BOV_103H23

0.0

XM_586821

IL2RB

Interleukin 2 receptor, beta

9.96

3.73E-05

2.52E-06

C0006005p10

e-171

NM_001102

ACTN1

Actinin, alpha 1

9.95

7.22E-08

2.14E-09

C0006020m09

e-131

NM_005726

TSFM

Ts translation elongation factor, mitochondrial

9.63

5.24E-11

4.79E-13

C0006012n18

e-101

NM_000362

TIMP3

TIMP metallopeptidase inhibitor 3

9.50

2.71E-05

1.76E-06

C0005202d9

0.0

XM_870158

ANKRD22

Ankyrin repeat domain 22

9.48

6.36E-17

3.79E-20

pIL1B

0.0

NM_000576

IL1B

Interleukin 1, beta

9.37

3.16E-13

1.07E-15

C0006020d11

0.0

XM_865242

ZFAND2A

Zinc finger, AN1-type domain 2A

9.28

1.10E-14

1.31E-17

C0005208c5

e-100

NM_002575

SERPINB2

serpin peptidase inhibitor, clade B (ovalbumin), member 2

9.25

3.92E-14

6.22E-17

C0005202i8

0.0

XM_583725

APOBEC3A

Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A

8.74

1.20E-13

2.97E-16

  1. Summary of the top 25 genes exhibiting increased mRNA abundance 16 hours post activation including clone name, accession number, e-value and identity of the best BLAST match and average fold change. The P and FDRmax values denote the probability from the moderated t-test and the Benjamini and Hochberg false discovery rate values respectively from the analysis of the resting cell values compared to the average activated sample values (Pre v Post).