Table 8 Details of the qRT-PCR primers and amplicons.
From: Development and validation of a bovine macrophage specific cDNA microarray
Gene | Gene Symbol | Clone | Direction | Sequence (5'-3') | Size (bp) |
|---|---|---|---|---|---|
CD9 antigen | CD9 | BE751736 | F | TTG GAC TAT GGC TCC GAT TC | 261 |
| Â | Â | Â | R | CCT TGA TCA CCT CCT CCT TG | Â |
CD44 antigen | CD44 | AJ816969 | F | TAC CCA CCC ACA AGA GAA CC | 102 |
| Â | Â | Â | R | AAC CCA TTA CCA GTC ATC CTT G | Â |
CD86 antigen | CD86 | pCD86 | F | GAA GGT CCC AAG GAT TGG TT | 136 |
| Â | Â | Â | R | GAT GAG CAG GTC AAA TTG ATG A | Â |
Chemokine (C-C motif) ligand 2 | CCL2 | AJ821161 | F | TCG CCT GCT GCT ATA CAT TC | 274 |
| Â | Â | Â | R | AGA GGG CAG TTA GGG AAA GC | Â |
Chemokine (C-C motif) receptor 1 | CCR1 | BE756795 | F | AAA TGA GAA GAA GGC CAA AGC | 140 |
| Â | Â | Â | R | TGC TCT GCT CAC ACT TAC GG | Â |
Complement component 1, r subcomponent | C1R | AJ818500 | F | AGA TTT GTC CGT CTG CCA GT | 183 |
| Â | Â | Â | R | CCC AGC GGT CAT TTC TCT TA | Â |
Fibronectin 1 | FN1 | AJ819936 | F | TAT CTT GGT GCC ATT TGC TC | 258 |
| Â | Â | Â | R | ATC CAC GCT TGT TTC TCT GG | Â |
Interleukin 6 | IL6 | pIL6 | F | TGA GTG TGA AAG CAG CAA GG | 289 |
| Â | Â | Â | R | CAG CAG GTC AGT GTT TGT GG | Â |
Prion protein | PRNP | AJ821011 | F | TGT GTA TGG TGG GAT TGG AA | 197 |
| Â | Â | Â | R | GCT GGG TCA TCA AAG AAA TG | Â |
V-fos FBJ murine osteosarcoma viral oncogene | FOS | BE755117 | F | CTT CTC CAG CAT GGG TTC TC | 257 |
| Â | Â | Â | R | TCT GAG CTC TGC CTC CTG TC | Â |
Chromosome 13 open reading frame 8 | C13orf8 | AJ817183 | F | AGC AGT GAC CAA GAG CAG GT | 205 |
| Â | Â | Â | R | TCA TAG CAC GAC AGC AAC AA | Â |