Skip to main content
Figure 4 | BMC Genomics

Figure 4

From: In silico and in vivo splicing analysis of MLH1 and MSH2 missense mutations shows exon- and tissue-specific effects

Figure 4

Splicing assay mimics in vivo alternative splicing and varies in human cell lines. (A) Results of the splicing assay following transfection with constructs harboring the different hMSH2 and hMLH1 normal exons. Cos-7 (C), Hep-3B (Hp) and HeLa (He) cells were transfected with 1 μg of the indicated wild-type exon, RNA was extracted, reverse transcribed, and amplified with primers SD6 and SA2. The RT-PCR products were resolved on GeneGel Excel, stained with ethidium bromide and quantitated with an image analyzer (see methods). Mw = size standard. The percentage of exon inclusion is indicated below each lane. (B) The graph shows the average of percent exon inclusion in the three different cell lines when transfected with the contructs containing the different normal exons (y-axis), representing the mean of two independent transfections done in triplicate for each construct (x-axis). Error bars represent standard deviation. The data were analysed using Student's t test (* = P < 0.05, ** = P < 0.01, *** = P < 0.001).

Back to article page