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Figure 3 | BMC Genomics

Figure 3

From: Molecular phylogeny of the antiangiogenic and neurotrophic serpin, pigment epithelium derived factor in vertebrates

Figure 3

Amplification of serpin sequences to verify the expression of genes identified in databases. RNA and cDNA were prepared from brain or cell lines from the species listed and amplified using primers listed in Table 4. All primer pairs correspond to different exons ensuring that amplicons arise from mature RNA. Lanes 1, 100 bp marker; 2, Frog E2; 3, Frog F1; 4, Zebrafish F1; 5, Chick F1; 6, Fugu F1; 7, Fugu F2; 8, Medaka F1;9, PigB2; 10, Pig C1; 11, Pig F1; 12, Pig F2, 13, Dog F1;14, 100 bp marker.

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