Figure 6

Structural models showing conservation of allergen and epitope structure between serine protease allergens and allergen homologues. A: View of protease Pen ch 18 predicted structure as a space fill model showing conserved epitope present in all fungi. B: Reverse view of Pen ch18 showing epitopes conserved in ascomycetes C: View of Pen ch 18 showing epitopes conserved in yeasts, C. albicans, C. tropicalis, S. cerevisiae and R, mucilaginosa. D: Reverse view of Pen ch 18 showing epitopes conserved in yeasts. E: Representative protease structures consisting of Pen ch 18, C. albicans homologue and C. cinereus models superimposed. F: Protease epitope structure for ascomycetes. The exposed epitope structure is displayed as ribbon and the buried portion of the epitope is shown as a blue CA trace. G: Protease epitope structure for basidiomycetes. The exposed epitope structure is displayed as ribbon and the buried portion of the epitope is shown as a red CA trace. H: Protease epitope structure for yeasts. The exposed epitope structure is displayed as ribbon and the buried portion of the epitope is shown as a yellow CA trace. I: Amino acid sequence pile up of the divergent loop in ascomycete epitopes (circled in B, E, F, G and H). Epitope regions are shown as a red bar underneath the pileup and surface exposed residues are shown by the blue bar. Allergen sequences are indicated by the red overlay.