Skip to main content

Table 1 Strains and plasmids. Strains and plasmids used in this study, including their origin, reference and main characteristics

From: TasA-tasB, a new putative toxin-antitoxin (TA) system from Bacillus thuringiensis pGI1 plasmid is a widely distributed composite mazE-doc TA system

Strains or plasmids

Relevant features

Construction, source or reference

Strains

  

   B. thuringiensis H1.1

Isolated from Ephestia kühniella, Canada

IEBC (International Entomopathogenic Bacillus Center, Pasteur Institute, Paris, France)

   E. coli TOP10

Cloning host

InVitrogen

   E. coli TG1

Cloning host

Gibson, 1984

Plasmids

  

   pGI1

Resident plasmid from B. thuringiensis H1.1

Mahillon and Seurinck, 1988

   pCR4-TOPO

Positive-selection cloning vector, AmpR, KanR

InVitrogen

   pCYB1

Cloning vector, AmpR, IPTG-inducible ptac promoter

New England Biolabs Inc.

   pCYB10

pCYB1 deleted of intein gene

J. Mahillon and F. Bilocq unpubl. results

   pBAD33

Cloning vector, CmR, arabinose-inducible promoter pBAD

Guzman et al., 1995

   pGIF02

PCR fragment containing antitoxin gene of pGI1 cloned into pCR4-TOPO

This study

   pGIF03

Nde I-Sal I pGIF02 fragment containing the antitoxin gene A cloned into pCYB1O

This study

   pGIF04Mx

PCR fragment containing the toxin gene P cloned into pCR4-TOPO, 4 clones obtained M1 → M4

This study

   pGIF06Mx

PCR fragment containing the antitoxin-toxin locus AP cloned into pCR4-TOPO, 4 clones obtained M1 → M4

This study

   pGIF08M1

PCR fragment containing the antitoxin-toxin locus pAP cloned into pCR4-TOPO

This study

   pGIF10Mx

PCR fragment containing the antitoxin-toxin locus p2AP cloned into pCR4-TOPO, 15 clones obtained M1 → M15

This study