Strains or plasmids | Relevant features | Construction, source or reference |
---|---|---|
Strains | Â | Â |
   B. thuringiensis H1.1 | Isolated from Ephestia kühniella, Canada | IEBC (International Entomopathogenic Bacillus Center, Pasteur Institute, Paris, France) |
   E. coli TOP10 | Cloning host | InVitrogen |
   E. coli TG1 | Cloning host | Gibson, 1984 |
Plasmids | Â | Â |
   pGI1 | Resident plasmid from B. thuringiensis H1.1 | Mahillon and Seurinck, 1988 |
   pCR4-TOPO | Positive-selection cloning vector, AmpR, KanR | InVitrogen |
   pCYB1 | Cloning vector, AmpR, IPTG-inducible ptac promoter | New England Biolabs Inc. |
   pCYB10 | pCYB1 deleted of intein gene | J. Mahillon and F. Bilocq unpubl. results |
   pBAD33 | Cloning vector, CmR, arabinose-inducible promoter pBAD | Guzman et al., 1995 |
   pGIF02 | PCR fragment containing antitoxin gene of pGI1 cloned into pCR4-TOPO | This study |
   pGIF03 | Nde I-Sal I pGIF02 fragment containing the antitoxin gene A cloned into pCYB1O | This study |
   pGIF04Mx | PCR fragment containing the toxin gene P cloned into pCR4-TOPO, 4 clones obtained M1 → M4 | This study |
   pGIF06Mx | PCR fragment containing the antitoxin-toxin locus AP cloned into pCR4-TOPO, 4 clones obtained M1 → M4 | This study |
   pGIF08M1 | PCR fragment containing the antitoxin-toxin locus pAP cloned into pCR4-TOPO | This study |
   pGIF10Mx | PCR fragment containing the antitoxin-toxin locus p2AP cloned into pCR4-TOPO, 15 clones obtained M1 → M15 | This study |