TILLING is an effective reverse genetics technique for Caenorhabditis elegans

BMC Genomics

Table 1 List of TILLING targets, sizes of amplicons and number and type of mutations identified for each gene.

Gene Name	Description	Gene Size (bp)	PCR Size (bp)	¹Prev. alleles	²Prev. strains	³Mis- sense	³Null	³Silent	³Total
*C05C10.5	Hypothetical protein	788	1175	0	0	2		1	3
mel-32 C05D11.11	Serine hydroxyl-methyl-transferase	1600	1500	16	1	4	1	2	7
mus-81 C43E11.2	Endonuclease MUS81	2530	1171	1	0	4		3	7
xpf-1 C47D12.8	Structure-specific endonuclease ERCC1-XPF	9112	1452	0	0	5		3	8
*F25H2.13	Helicase of the DEAD superfamily	4985	1499	1	0	5		4	9
htp-3 F57C9.5	HIM-3 paralogue	2598	1452	1	1	5		5	10
*M03C11.2	Helicase of the DEAD superfamily	5943	1490	1	0	4		1	5
cki-2 T05A6.2	Hypothetical protein	1555	1569	0	0	7	1	2	10
mdf-2 Y69A2AR.30	Spindle assembly checkpoint protein	4461	1466	1	0	1		5	6
htp-2 Y73B6BL.2	HIM-3 paralogue protein 2	1199	1451	0	0	5		1	6
Totals			14225			42	2	27	71

* Gene name not assigned
¹ Number of mutant alleles listed in Wormbase [7] as existing prior to this study.
² Number of mutant strains available from the Caenorhabditis Genetic Stock Center.
³ Number of mutations of this type identified in this TILLING study.
Missense mutations alter the amino acid sequence of the encoded protein. Null mutations refer to mutations that convert an amino acid codon into a premature stop codon, or that alter a conserved splice junction and result in premature truncation of the protein product of the gene. Silent mutations are changes that do not affect the protein product of the gene. These include mutations in introns or intergenic sequences, and mutations that alter the third bp of a codon in such a way that it does not change the amino acid encoded by that codon.

ISSN: 1471-2164