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Table 2 Sequence coverage of four BAC clones from two independent sequencing experiments using 454 sequencing technology

From: 454 sequencing put to the test using the complex genome of barley

BAC

size (bp)

total readsa

avg. size (bp)b

contigs

contig size (bp)

coverage

773K14

113.510c

59.126e

101

65

109.444

52.7 ×

  

6.108f

104

210

106.476

5.6 ×

519J4

102.554c

32.564e

102

94

75.526

32.7 ×

  

4.917f

102

209

72.634

4.9 ×

604D5

110.000d

70.510e

103

97

101.195

66 ×

  

9.683f

103

137

102.468

9.1 ×

509D2

120.000d

19.208e

105

80

100.062

16.8 ×

  

3.801f

104

302

66.647

3.3 ×

  1. aSequences containing BAC vector or E. coli were removed.
  2. bAverage read length of 454 sequences.
  3. cPreviously published, exact size is known.
  4. dEstimated by gel electrophoresis.
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BMC Genomics

ISSN: 1471-2164

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