Table 5 List of used primers, their temperature and MgCl optimum and length of product.
name | direction | sequence | size | MgCl μmol | annealing temp |
|---|---|---|---|---|---|
GAPDH | F | ATG CCA GTG AGC TTC CCG TTC AGC | 199 | 2 | 70 |
GAPDH | R | TGG TAT CGT GGA AGG ACT CAT GAC | |||
VRK2 | F | GCAGAAAGAGGAGAAACTGATTGGA | 245 | 4 | 62 |
VRK2 | R | CCGTGCTGACTGTGGAAGTGTATT | |||
GM2A | F | TTCCTTGCCACTGTCCCTTCA | 222 | 3 | 61 |
GM2A | R | CTTCCTCACACCGCTCCATTCT | |||
CRYAB | F | CCCCTTCTTTCCTTTCCACTCC | 266 | 3 | 62 |
CRYAB | R | CACCTCAATCACATCTCCCAACAC | |||
DRPLA | F | GAACTCTCCCTAACCCCCTGCTT | 279 | 3 | 63 |
DRPLA | R | GTGGCTTGTCGCTTTCCTTCTTC | |||
DIO2 | F | GGGCATCCTCAGCGTAGACTTG | 295 | 4 | 64 |
DIO2 | R | GCCACTGTTGTCACCTCCTTCTGT | |||
PSMB | F | TATTTATTGTTGTGGTGCTGGGACA | 350 | 4 | 65 |
PSMB | R | TCTTGGCTTCCTCCTCCTCCA | |||
ROK1 | F | CCTGTTCTTGTTTTTGTTCAGTCCA | 298 | 3 | 61 |
ROK1 | R | TTGCTTTTCCCTTATTCCCTGCTC | |||
PLCG1 | F | CTCAACTTCCAGACCCCTGACAA | 237 | 3 | 64 |
PLCG1 | R | CACCTCAATCTCCACAAAAGGACAC | |||
KRT19 | F | GCACCCTTCAGGGTCTTGAGAT | 346 | 3 | 64 |
KRT19 | R | AAGACACCCTCCAAAGGACAGC |